Yes - but you need to avoid conflicts with shortcut keys that the web browsers you support use.
Generally some input element in your VLF-ONE application needs to have focus for a shortcut key to function as you expect.
To enable a shortcut key globally see the shipped example DF_OSYSTM for an example of globally enrolling and actioning shortcut keys.
To enable a shortcut key in a command handler or filter use code like this:
Define_Com Class(#PRIM_STPG) Name(#CtrlF7) Parent(#COM_OWNER) Shortcut(Ctrl+F7)
#Sys_web.Alert Caption('Ctrl-F7 used')
Also refer to the Example Applications -> Advanced -> Short Cut and Function Keys.